Curcumin-primed exosomes potently ameliorate cognitive function in AD mice by inhibiting hyperphosphorylation of Tau protein through the AKT/GSK-3β pathway

摘要：

The toxic activity of cur on macrophage RAW264.7 cell was determined in a 24 hourMTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazoliumbromide) assay (cytotoxicity test) and we found that the cell viability of cur was not significantly decreased at all concentrations, ranging from 5 to 40 μg/mL, and more than 80% of cells survived. Based on these results, macrophage RAW264.7 cells were treated with cur (40 μg/mL) for 24 h. Culture media was collected and centrifuged at 3,000 rpm (10 min) and supernatant was collected. Collected supernatant was further